Fig. 4

Cisplatin induced NF-κB translocation to nucleus especially in OCT4 positive cells: a Immunofluorescence for NF-κB (Red, first panel) and OCT4 (green, second panel) pre and post cisplatin treatment in MP, SP and NSP of A2780-CisR. Third (merged) and fourth (zoomed image) panel represents overlap of all three channels (blue-DAPI). (Scale-10 μm) b-d Quantification of NF-κB revealed significant induction in nuclear NF-κB only in MP (b), and SP (c) but not in NSP (d) upon cisplatin treatment. Cisplatin did not altered percentage of OCT4+ cells in all three fractions, however, it dramatically increased dual (NF-κB+ and OCT4+) cells in SP (c). Nuclear fluorescence for NF-κB (Red) and OCT4 (Green) was quantified from minimum of ~100 cells and represented as percentage ± SEM of single or dual staining. Also see Additional file 6: Figure S5