Fig. 5

ID1 regulates G6PD transcription through c-MYC activation. a G6PD transcriptional activity was measured by dual luciferase reporter assay. Relative luciferase activity was normalized to the luciferase activity of control vector. As indicated, transcriptional activity of G6PD promoter was downregulated by silencing ID1 expression and was recovered upon c-MYC plasmid expression (P = 0.01). b Illustration of potential c-MYC binding sites on G6PD promoter, predicted by bioinformatic analysis. c Schematic representation of luciferase reporter constructs #1(−2000, +1), #2 (−1500, +1) and #3 (−1000, +1). d Dual luciferase assay of HEK293T cells co-transfected with plasmids containing different G6PD promoter reporter constructs with or without c-MYC plasmid. e Dual luciferase reporter assay of HEK293T cells co-transfected with pGL3-G6PD-WT or pGL3-G6PD-Mut (GGATATAAAC) constructs, together with c-MYC expression plasmid. Results shown are mean ± SD of three experiments