Fig. 3

Knockdown of ALX4 in MDA-MB-231-ALX4 and MCF-7-ALX4 stably cells recovered the inhibition effect. a Knockdown of ALX4 in MDA-MB-231-ALX4 and MCF-7-ALX4 stably cells was identified by WB and RT-PCR. b MTS assays were used to examine the effect of ALX4 knockdown on the relative number of viable cells based on the absorbance. c Knockdown of ALX4 recovered the colony formation ability. d Transwell assays were performed to examine the effect of ALX4 knockdown on cell migration and invasion. Error bars indicate s.d. (n = 3).* P < 0.05; ** P < 0.01