Fig. 7

Clinical relevance of miR-141-3p with TRAF5, TRAF6 and NF-kB signaling activity in human PCa and bone tissues. a Analysis of miR-141-3p expression with TRAF5, TRAF6 and nuclear p65 in 4 non-bone metastatic PCa tissues (T1–4) and 4 bone metastatic PCa tissues (T5–8). U6 was used as the control for RNA loading. miR-141-3p expression levels were normalized to that miR-141-3p expression of sample one. Each bar represents the mean ± SD of three independent experiments. Loading controls were α-tubulin and p84 for the cytoplasmic and nuclear fractions. b-d Correlation between miR-141-3p levels and TRAF5, TRAF6 and nuclear p65 expression in PCa and bone tissues.The expression levels of TRAF5, TRAF6 and nuclear p65 were quantified by densitometry using Image J Software, and normalized to the levels of α-tubulin and p84, respectively. The sample 1 was used as a standard. The relative expressions of miR-141-3p and these proteins were used to perform the correlation analysis