Fig. 3

Knockdown of PSAT1 inhibited tumorigenicity of ER-negative breast cancer cells. a Western blot shows PSAT1 expression in HCC70 and MDA-MB-468 cells infected with Lenti-shPSAT1 or control. β-tubulin was used as a loading control. b CCK-8 assay was performed to determine the effect of PSAT1 silencing on the proliferation of the indicated cells at the indicated time points. c Knockdown of PSAT1 suppressed the colony formation ability of HCC70 and MDA-MB-468 cells compared with that of control cells. The values of the control cells were normalized to 1. For (b) and (c), the results are expressed as the mean ± SD; n = 3. d Cell cycle analysis of the indicated cells according to flow cytometry. *p < 0.05. **p < 0.01, ***P < 0.001