Fig. 6

PSAT1 regulated Cyclin D1 expression through GSK3β/β-catenin. a The levels of cyclin D1 mRNA were analyzed by qRT-PCR in the indicated cells. The values of the NC and Vector groups were normalized to 1. b The protein expression levels of β-catenin, total GSK-3β, p-GSK-3β (Ser9) and cyclin D1 were examined by western blotting in the indicated cells. c Western blotting was performed in conjunction with cell fractionation to analyze the nuclear translocation and expression of β-catenin in the indicated cells. **P < 0.01, ***P < 0.001, ****P < 0.0001