Fig. 3

Increased migration of cancer cells and PSCs. a Increased migration of cancer cells out of the spheroid channel. Representative images of PKH26-labelled cancer cells migrating into the media channel are shown at days 6, 8, and 10. b Increased migration of PSCs (stained with F-actin) towards the tumor spheroids compartment after 5 days of culture. The location of PSCs was determined using nucleus staining. Optical sections were acquired at 6 μm intervals and stacked into a z-projection. Data are expressed as the mean ± SE of 3 independent experiments. Scale bars, 100 μm; Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. PSCs, pancreatic stellate cells; TS, tumor spheroids