Fig. 6

Differential sensitivity of PANC-1 tumor spheroids to anti-cancer drugs. Cells were grown for 5 days and stained for viability (calcein AM/PI) after 72 h exposure to gemcitabine (a), combination of gemcitabine and paclitaxel (b). Optical sections were acquired at 6 μm intervals and stacked into a z-projection from which fluorescence intensity was calculated. Data are expressed as the mean ± SE of 3 independent experiments. Scale bars 100 μm. Student’s t-test, *p < 0.05, ** p < 0.01. PSCs, pancreatic stellate cells; TS, tumor spheroids