Fig. 2
From: Inhibition of EGR1 inhibits glioma proliferation by targeting CCND1 promoter
Targeting EGR1 by RNA interference inhibited the proliferation of glioma cells. a Real-time quantitative PCR analysis of EGR1 in U251, U251SLC, and U87 cells transfected with control siRNA vector and siEGR1-vector. b Immunoblot analysis of EGR1 in U251, U251SLC, and U87 cells transduced with control vector and siEGR1-vector. c, d The rates of cell growth were detected by CCK8 assay in U87 and U251 cells transfected with control siRNA or EGR1 siRNA. e Cell proliferation rates as determined by EDU assay in U87, U251 and U251SLC cells transfected with control siRNA or EGR1 siRNA. f Cell cycle analyzed by propidium iodide staining and flow cytometry in U87, U251 or U251SLC cells transfected with control siRNA or siRNA targeting EGR1. (At least three repeated experiments for the all cell types). *P < 0.05, **P < 0.01, and ***P < 0.001 (mean ± SEM)