Fig. 2
From: Diagnosis and treatment of ALT tumors: is Trabectedin a new therapeutic option?
a. Response to G-quadruplex ligands and Trabectedin treatment in ALT and non ALT cell lines. ALT positive (SK Br3, U2OS, SW872, SW982, MG63, Saos2, M20) and negative (HCT116, H1299, HeLa HT29, HT1080, HOS, M14) cell lines were treated with Emicoron (dose range from 0,2 to 1,4 μM), CX-5461 (dose range from 0,01 to 1 μM) and Trabectedin (dose range from 0,15 to 2,4 nM) for 120 h, and then fixed and processed for crystal violet staining. Surviving fraction of cells was determined as percent of treated vs untreated absorbance values in each condition. Then the IC50 dose (the dose able to kill the 50% of cells compared to untreated sample) was calculated by Calcusyn software. The IC50 doses of compounds were compared in the ALT- and ALT+ groups of cell lines and results are shown in the box and whiskers plot (** = P < 0,005). b. Correlation between IC50 doses of Trabectedin and the indicated parameters (black: ALT-; red: ALT+). The “Pearson r” coefficient of correlation was calculated by the GraphPad Prism 7.03 software