Fig. 2

Inhibition of linc-ROR decreases CSC-like properties. a CCK8 assay revealed a time-dependent inhibition of cell proliferation after linc-ROR siRNA treatment compared with scramble control (NC) in EC9706 cells. b Reduced colony-forming capacity of EC9706 cells after silencing of linc-ROR. c Effect of linc-ROR knockdown on cell migration (top) and invasion (bottom) of EC9706 cells assessed using Transwell assay. d Immunoblot analysis of E-cadherin and vimentin expression in EC9706 cells after treatment with linc-ROR siRNA. β-actin was used as a loading control. e Cell viability of EC9706 after treatment with scramble or linc-ROR siRNA was determined by CCK8 assay in the presence of indicated doses of cisplatin. f Representative images of tumorspheres (top) and quantification of tumorsphere numbers (bottom) formed by Eca109 cells (per 1000 cells) after linc-ROR knockdown in the presence or absence of cisplatin (Cis; 3 μg/mL). g qRT-PCR analysis of stemness-associated genes CD44, KLF4, NANOG, OCT4, and SOX2 expression in EC9706 cells following linc-ROR knockdown. Transcription levels were normalized to GAPDH expression