Fig. 1
5-FU resistant pancreatic cancer cells are hypersensitive to PCT. a All cell lines were treated with PCT (TPCS2a + light) with increasing light exposure up to 240 s. Reduction in cell viability (%) relative to untreated cells was measured 72 h after light exposure by MTS assay. The experiments were repeated more than five times, representative data are shown. Error bars represent standard deviation. Statistically significant difference between the effect of PCT in the 5-FU sensitive vs the 5-FU resistant cell lines (P < 0.01) is indicated by *. b and c Cellular uptake of TPCS2a (red) and Lysotracker (LTG, green) in Panc03.27S–Nt and Panc03.27R–B1V cells, following b no light activation, and c 60 s light activation (white arrows are included to show remaining LTG signal in Nt cells). Co-localization of TPCS2a (red) and LTG is indicated by yellow fluorescence. 10 μg/ml Hoechst 33342 and 50 nM LTG was added 15 min and 30 min prior to image acquisition, respectively. The scale bar is 20 μm