Fig. 8
PCI-triggered endosomal escape of CD105-saporin induces specific and efficient cytotoxicity. a and b Cellular uptake of CD105–488 and TPCS2a in a Panc03.27S–Nt and b Panc03.27R–B1V. Co-localization of TPCS2a (red) and Lysotracker (green) is indicated by yellow fluorescence. 10 μg/ml Hoechst 33,342 and 50 nM LysoTracker were added 15 min and 30 min prior to image acquisition, respectively. The scale bar is 20 μm. c and d 5-FU sensitive (c) and 5-FU resistant (d) cell lines were subjected to either PCT, PCI of CD105-saporin, or PCI of saporin, following the PCI protocol described in Methods. Reduction in cell viability (%) relative to untreated cells was measured 72 h after light exposure, by MTS assay. All treatments are listed in the inserted table. The experiments were repeated at least 4 times, representative data are shown. Error bars represent SD. Statistically significant difference between PCI of CD105-saporin and PCI of saporin at the different time points is indicated by * and the indicated P-value