Fig. 7
The effects of Notch1 on tumor-induced immunosuppression were attributed to the upregulation of TGF-β1. a Relative mRNA expression of TGF-β1, IL-10 and VEGF in Notch1 knockdown or overexpressing B16 cells. b TGF-β1, IL-10 and VEGF secretion levels in culture supernatant of Notch1 knockdown or overexpressing B16 cells were determined by ELISA. c The apoptosis of CD3+ T lymphocytes were analyzed in TGF-β1 neutralization test. d CD3+ T lymphocytes labeled with CFSE were analyzed for proliferation after anti-TGF-β1 administration. e In vitro cytotoxicity of CD8+ T lymphocytes against Notch1 knockdown or overexpressing cells after TGF-β1 was neutralized. f Secretions of IFN-γ and immunosuppressive cytokines TGF-β1, VEGF and IL-10 in homogenate supernatant of tumor tissues were determined using ELISA. 8 mice per group were used in this experiment. *, P < 0.05