Fig. 3

MCM6 promotes EMT through activating MEK/ERK signaling. a-b Analysis of the mRNA and protein levels of EMT-associated genes shows that MCM6 expression is inversely correlated with E-cadherin but positively correlated with vimentin expression. The other EMT markers were not obviously influenced by the change in MCM6. c Representative IF images of E-cadherin and vimentin expression in the cells treated with LV-shMCM6 or LV-Scramble. Scale bars: 20 μm. d Expression of MCM6, p-ERK, p-MEK, ERK, and MEK was detected by western blot. e Immunofluorescence confirmed that MCM6 participates in the nuclear expression of ERK. Scale bars, 20 μm