Fig. 4

EZH2 expression was inversely correlated with miR-22 expression, and EZH2 regulated galectin-9 via miR-22. a EZH2 expression was inversely correlated with MIR22HG expression in clinical HCC specimens (n = 498) derived from the R2: microarray analysis and visualization platform (http://r2.amc.nl). b qPCR showed that miR-22-5p expression levels were inversely correlated with EZH2 transcript levels in HCC tissues (n = 24). c qPCR revealed that miR-22 expression was inversely correlated with EZH2 expression in HCC cells. d miR-22 and MIR22HG levels were measured after EZH2 knockdown and overexpression and IFN-γ treatment in HepG2 and Hep3B cells. e miR-22 and MIR22HG levels were measured after EZH2 overexpression and IFN-γ treatment in HepG2 and Hep3B cells. f Schematic representation of the MIR22HG promoter region. g The miR-22 promoter was dissected, as shown schematically, and the luciferase activitylevels of different constructs were determined in HCC cell lines. h HCC cells were stably transfected with EZH2 shRNA or pCMV-EZH2 plasmids, and ChIP assay was conducted to determine EZH2 and H3K27me3 enrichment within the MIR22HG promoter area (* P < 0.05, **p < 0.01, compared to mimic-NC, inhibitor-NC, or mock)