Fig. 5

miR-22 and galectin-9 suppressed HCC cell proliferation, migration and invasion in vitro. a Western blot showing galectin-9 levels in cells transfected with mock or pre-miR-22 and cells co-transfected with galectin-9. b Growth curves of HCC cells transfected with the indicated vectors. c Apoptosis rates were measured by AnnexinV-PE and 7-AAD staining flow cytometry in cell with enforced expression of miR-22 and galectin-9. d Levels of caspase3, BCL2, BAX were detected by Western blot. e, Representative images and results of colony formation assay (magnification ×100; scale bar, 1 cm) of HCC cells transfected with the indicated vectors. f Representative images (magnification ×100; scale bar, 100um) and results of transwell migration(top) and Matrigel invasion(bottom) assays of HCC cells transfected with the indicated vectors.(* P < 0.05, ** P < 0.01,compared to mock)