Fig. 2

SMURF1 depletion decreases the expression of ER alpha target genes in breast cancer cells. a Top 10 signaling pathways significantly decreased by SMURF1 depletion in MCF7 cells. The pathway-enrichment analysis was used by the threshold P < 0.001 and fold change > 2 to derive regulated genes. SMURF1 was depleted by siRNA (mix of siSMURF1 #1 and siSMURF1 #2) or treated with siControl. After 48 h, the whole mRNA was extracted for RNA sequence analysis. The siControl and siSMURF1 were done in triplicates. b Top 10 signaling pathways, which were significantly activated by SMURF1 depletion in MCF7 cells. The pathway-enrichment analysis was used by the threshold P < 0.001 and fold change > 2 to derive regulated genes. The pathway-enrichment analysis was used by the threshold P < 0.001 and fold change > 2 to derive regulated genes. SMURF1 was depleted by siRNA (mix of siSMURF1 #1 and siSMURF1 #2) or treated with siControl. After 48 h, the whole mRNA was extracted for RNA sequence analysis. The siControl and siSMURF1 were done in triplicates. c The heat-map graph shows the ERα regulating genes, which is significantly changed by SMURF1 depletion in MCF-7 cells