Fig. 4

Autophagy activated Wnt/β-catenin signaling in HCC cells. (a, b) Western blot showed the expression of β-catenin and p-β-catenin in the starved HepG2 and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h. (c, d) Immunofluorescence showed the location of β-catenin in starved HepG2 cells and SMMC-7721 cells cultured with control, EBSS or EBSS + 3MA for 6 h. Data are shown as the mean ± SEM of three independent experiments. *p < 0.05