Fig. 5

β-Catenin downregulation inhibited autophagy-induced glycolysis in HCC cells. (a, b) Real-time PCR and western blot were used to examine the expression of β-catenin in HepG2 and SMMC-7721 cells transfected with β-catenin siRNA. (c) The expression of MCT1 in HepG2 cells and SMMC-7721 cells cultured with EBSS for 6 h after transfected with β-catenin siRNA. (d-g) Analysis of the consumption of glucose and production of lactate in HepG2 cells and SMMC-7721 cells cultured with EBSS for 6 h after transfected with β-catenin siRNA . Data are shown as the mean ± SEM of three independent experiments. (h, i) The effect of activation of autophagy with EBSS on Wnt/β-catenin signaling pathway was detected by dual-luciferase reporter assays in HepG2 cells and SMMC-7721 cells. *p < 0.05