Fig. 3
From: INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia
INPP4B mediates SGK3 activation in OCI-AML3 cells. a Western blotting analysis of INPP4B, p-SGK3T320, SGK3, p-AKTT308 and AKT from OCI-AML3 cells transduced with the control siRNA or siINPP4B. b qRT-PCR analysis of INPP4B mRNA expression from OCI-AML3 and THP-1 cells transduced with the pEAK-Flag/INPP4B plasmids. c-d Western blotting analysis of Flag, INPP4B, p-SGK3T320, SGK3, p-AKTT308 and AKT from the INPP4B transduced cells. e Western blotting analysis of p-SGK3T320, SGK3, p-AKTT308 and AKT from the OCI-AML3 cells treated with LY294002 (30 μM), MK-2206 (5 μM) and Rapamycin (5 μM), respectively. Proteins were quantified using image software and normalized against β-actin. The relative abundance of (f) PI (3,4) P2 and (g) PI (3) P in the INPP4B transduced cells was measured using ELISA. Data were represented as mean ± s.d. of three individual experiments. * p < 0.05