Fig. 1

Tetrandrine inhibits HCC cell invasion and migration. a Data are presented as the mean ± SD of at least three independent experiments, *p < 0.05. b Transwell migration assay was conducted with Huh7, HCCLM9, and Hep3B cells treated with DMSO or 2-μM tetrandrine (Tet) for 24 h. The experiment was repeated at least three times, **p < 0.01. c The invasive properties of Huh7 and HCCLM9 cells treated with DMSO or 2-μM tetrandrine (Tet) for 48 h were assessed using a transwell invasion assay with a matrigel invasion chamber. The experiment was repeated three times, *p < 0.05. d Immunofluorescence staining for F-actin (using rhodamine phalloidin) in Huh7 and HCCLM9 cells after treatment with DMSO or 2-μM tetrandrine (Tet) for 24 h. Scale bars: 20 μm. e Anoikis assay. FACS analysis for anoikis after DMSO or 2-μM tetrandrine (Tet) treatment of Huh7, HCCLM9 and Hep3B cells for 72 h