Fig. 3

Characterization of CP-EPS8-NLS, a specific peptide that localizes to the nucleus. a Graphical representation showing the sequences of CP-EPS8-NLS, mutated CP-EPS8-NLS, and penetratin. b HL-60, NB4, THP-1, U937, TF1α and KG1α cells were treated for 24 h with increasing concentrations of CP-EPS8-NLS analyzed by CCK-8. c HL-60, THP-1 and U937 cells were seeded and treated with increasing concentrations of CP-EPS8-NLS for 2 weeks. Colonies were counted, and images were taken. d PBMCs from 5 unrelated healthy donors were treated with increasing concentrations of CP-EPS8-NLS for 24 h, and cell viability was analyzed via CCK-8 assay