Fig. 1

TFAP2C is upregulated in CRC tissues and cells. a TFAP2C expression level was elevated in colorectal cancer tissues compared with the adjacent normal tissues as assessed by analyzing the TCGA colorectal cancer RNA sequencing dataset (Adjacent normal tissue, n = 50; Colorectal cancer, n = 625). b TFAP2C expression level was elevated in 50 paired colorectal cancer tissues compared with the adjacent normal tissues as assessed by analyzing the TCGA colorectal cancer RNA sequencing dataset. c and d Real-time PCR analysis and Western blotting of TFAP2C expression in 8 paired CRC tissues. α-Tubulin was detected as a loading control in the Western blot and GAPDH was used as endogenous controls in RT-PCR. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05. e and f Real-time PCR and Western blotting analysis of TFAP2C expression in one normal colon epithelial cell CMEC and 7 CRC cell lines. GAPDH was used as endogenous controls in RT-PCR and α-Tubulin was detected as a loading control in the Western blot. Each bar represents the mean values ± SD of three independent experiments. *P < 0.05