Fig. 6

TFAP2C inactivates Hippo signaling pathway in CRC cells. a Representative immunofluorescent images of CRC cells were immunostained with YAP antibody (red) or phalloidin (green) in the indicated CRC cells. b and c The percentage of nuclear YAP+ (b) and nuclear TAZ+ (c) cell number via immunostaining in the indicated groups. *P < 0.05. d TEAD transcriptional activity was assessed by HOP-Flash luciferase reporter in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. e Western blotting of p-MST1/2, MST1/2, p-LATS1, LAST1, p-YAP, YAP and TAZ expression in the indicated cells. α-tubulin was used as the loading control. f Cellular fractionation and western blotting analysis of nuclear YAP and TAZ expression in the indicated cells. The nuclear protein p84 was used as the nuclear protein marker. g Real-time PCR analysis of CTGF, CYR61, HOXA1, SOX9, RPL13A and PPIA in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05