Fig. 4

DUXAP10 down-regulation inhibits GC cells tumor growth in vivo, and invasion in vitro. a Representative images of tumors formed in nude mice injected subcutaneously with DUXAP10 knockdown BGC823 cells, and the tumor growth curves of DUXAP10 down-regulation and control groups. b Tumors induced by DUXAP10 knockdown in BGC823 cells showed markedly lower weight than control cells. c Tumors developed from sh-DUXAP10 transfected BGC823 cells showed lower ki67 protein levels than tumors developed by control cells. Up: H & E staining; Down: immunostaining. d,e Transwell assays were used to investigate the changes in migratory and invasive abilities of DUXAP10 knockdown cells. f E-cadherin, N-cadherin, Vimentin and β-catenin protein levels were detected by western blot and Immunofluorescence analysis after DUXAP10 knockdown in BGC823 cells. *P < 0.05, **P < 0.01