Fig. 5

Effect of dacomitinib on the expression of ABCG2 in MDR cells. The protein level of ABCG2 on MDR cells after 0, 0.25, 0.5, 1 and 5 μM dacomitinib stimulation for 48h were measured by western blot analysis, and mRNA level were measured by PCR (GAPDH as loading control) (a). Dacomitinib did not alter the mRNA and protein levels. Real time-PCR was further applied to confirm the mRNA expression levels in MDR cells in the presence or absence of dacomitinib (b). The cell surface expression of ABCG2 were measured by flow cytometry in the presence or absence of dacomitinib in MDR cells and their parental cells (c). All these experiments were repeated at least three times, and representative images and densitometry results were shown in each panel. Columns, means of triplicate determinations