Fig. 3
CLCA2 influences NPC cell migration, invasion and overexpressing CLCA2 impairs NPC metastasis in vivo. a-b Overexpression of CLCA2 in NPC cells could inhibit cell migration and cell invasion in 5-8F and S18 cells compared with vector control cells. c-d Quantification of the effects of CLCA2 overexpression on the migratory abilities and invasion ability in 5-8F and S18 cells as determined by transwell assays, all of the experiments were performed at least three times. Columns, average of three independent experiments; bars, SD. *P < 0.05, **P < 0.01, result of Student t test. e Overexpression of CLCA2 decreased in vivo metastatic rate of S18 cells. Histological image of lung metastasis in nude mice after tail vein injection of S18 cells. Left, representative picture of lungs; right, representative H&E staining of lungs; scale bar, 50 μm. f The numbers of lung metastases (mean ± SD) were counted and summarized. *P < 0.05. g The metastasis rates decreased of overexpression of CLCA2 in S18 cells in vivo, cells (2 × 105) were subcutaneously injected into the left hind footpad of nude mice, and metastasis to the left popliteal LN was measured. h The primary tumor weights of left hind footpad are also compared with the control group. P > 0.05, Student t test. i The proportion of popliteal lymph node metastases was significantly reduced overexpression of CLCA2 in S18 cells (n = 36 in vector group, n = 37 in CLCA2 group). P values were calculated using a chi-square test