Fig. 4

Silencing CLCA2 promotes NPC cell migration and invasion in vitro and CLCA2 enhances the suppressive function chloride channel inhibitor on NPC cell migration. a-b Quantification of the effects of CLCA2 silencing on the migratory abilities of S26 and SUNE-1 cells as determined by transwell migration assays. Columns, average of three independent experiments; bars, SD. *P < 0.05, **P < 0.01, Student t test. c-d Quantification of the effects of CLCA2 silencing on the invasion abilities of S26 and SUNE-1 cells as determined by transwell invasion assays. Columns, average of three independent experiments; bars, SD. *P < 0.05, **P < 0.01, Student t test. e-h Chloride channel inhibitor (NFA) impaired the migration of high-metastasis cells. Cells were treated with 50 μM/L and 100 μM/L NFA or DMSO for 24 h and subjected to migration assays. The quantitative results of migrated cells were calculated by 3 visual fields selected in the transwell assay, means ± SD, n = 3, *P < 0.05;**P < 0.01, NS, P > 0.05, by student t test, scale bar, 100 μm