Fig. 3

14, 15-EET promotes tumor cells invasion and activates FAK/PI3K/AKT signaling through integrin αvβ3. MCF-7 and MDA-MB-231 cells were transfected with integrin αv or β3 siRNA or control siRNA. a The integrin αv or β3 expression was examined by Western blot. The integrin αv or β3 knockdown tumor cells were treated with 14, 15-EET (100 nM). b and c The phosphorylated and un-phosphorylated FAK, PI3K and AKT were detected by Western blot. d The invasive migration assay was performed. *p < 0.05, **p < 0.01