Fig. 7

APG-1387-induced autophagy promotes cell survival in SKOV3 cells. a, b, c The cell death was clearly evident in all cell lines by phase-contrast microscopy, Cells were incubated for 2 h in the presence or absence of 3-MA (20 mM), Baf A1 (100 nM) and CQ (10 μM), then, APG-1387 combined with or without 3-MA (20 mM), Baf A1 (100 nM) and CQ (10 μM) for 24 h. d, e, f Cells were incubated for 2 h in the presence or absence of 3-MA, Baf A1 and CQ, then, Cell viability were tested after APG-1387 combined with or without 3-MA, Baf A1 and CQ treatment 24 h was determined by the CCK-8 assay. g, h, i The expression of caspase-3 was measured by western blot. Cells were incubated for 2 h in the presence or absence of 3-MA, Baf A1 and CQ, then, APG-1387 combined with or without 3-MA, Baf A1 and CQ for 24 h. The data shown are representative of three different experiments