Fig. 4

WTAP regulated CDK2 expression in RCC cells and correlated with CDK2 expression in human RCC tissues. a, b) Western blot analysis of CDK2, CDK4 and CDK6 expression in Caki-1 and ACHN cells with WTAP knockdown or overexpression. CDK2 expression was obviously decreased in WTAP-knockdown cells whereas increased in WTAP overexpression cells. c IHC analysis of WTAP and CDK2 in RCC tissue microarray at 200× magnifcation. WTAP and CDK2 were both mainly expressed in the nucleus. d WTAP positive RCC expressed high level of CDK2; WTAP negative RCC expressed low level of CDK2. Scale bars indicate 100 μm