Fig. 2
SCD1 promotes migration and invasion of colorectal cancer cells by regulating EMT. a Representative Western blot of SCD1 and quantification result in five colorectal cancer cell lines. b Protein levels of SCD1 in HCT116 cells transfected with shRNAs for SCD1 (sh1 and sh2) or Caco2 cells ectopically expressing SCD1. c Transwell assay of HCT116 cells after SCD1 knockdown. The scale bar is 100 μm. d, e Histograms show the numbers of migrated (d) and invasive (e) HCT116 cells. f Transwell assay of Caco2 cell lines after SCD1 overexpression. The scale bar is 100 μm. g Histograms show the numbers of migrated and invasive Caco2 cells. h Morphological analysis of lung metastasis in mice injected with HCT116 cells transfected with control shRNA or SCD1 shRNA (n = 8 per group). Arrows indicate metastatic nodules in the lungs. The scale bar is 200 μm. i, j Protein levels of E-cadherin and vimentin in colorectal cancer cells transfected with SCD1 shRNA (i) or SCD1 cDNA (j)