Fig. 2
From: TNFα induces Ca2+ influx to accelerate extrinsic apoptosis in hepatocellular carcinoma cells
Ca2+ influx induced by TNFα was mediated by TRP channels and independent of TNF Receptors. a and e Confocal microscope analysis of [Ca2+]c using fluorescent probe Fura-2/AM in SNU739 and HLF cells with treatment as indicated. siTNFR1: siRNA targeted to TNFR1; siTRPM7: siRNA targeted to TRPM7. b and c Confocal microscope analysis of [Ca2+]c using fluorescent probe Fura-2/AM in SNU739 and HLF cells with treatment as indicated for 30 min before stimulation of 100 ng/mL TNFα. Diltiazem: 10 μM; Verapamil:40 μM; CAI: 10 μM; SKF96365: 100 μM. d The relative mRNA expression level of TRP channels in SNU739 and HLF cells. Data were shown as mean ± SD. All experiments were performed at least three times. ** P < 0.01