Fig. 2

MiR-1468 promotes cell proliferation, cell cycle progression, colony formation and inhibits apoptosis in HCC cells in vitro. a Hep3B and MHCC-97 L cells that were transfected with corresponding miRNA vectors were subjected to qRT-PCR for miR-1468 expression. Overexpression of miR-1468 promoted cell proliferation (b, c), colony formation (d), cell cycle progression (e) and inhibited apoptosis (f) in Hep3B cells, while down-regulation of miR-1468 inhibited cell proliferation (b, c), colony formation (d), cell cycle progression (e) and promoted apoptosis (f) in MHCC-97 L cells. g Western blot analysis of cycle regulator Cyclin D1 and p21, apoptosis-related protein Bcl2/Bax expression in the presence and absence of miR-1468. n = six independent experiments. *P < 0.05, **P < 0.01