Fig. 1

Galectin-1 is expressed in different BP-ALL subtypes. a Meta-analysis of GSE28497 representing 270 ALL diagnosis samples and four CD19 + CD10+ control normal B-cell precursors (normal BM). ALLs are subdivided into categories based on karyotype abnormalities as indicated [18] The category MLL includes BP-ALLs with different MLL gene rearrangements (bars, mean ± SEM; ***p≤0.001, 95% CI, 1-way ANOVA; other comparisons, ns.). Each symbol represents one sample. MFI, mean fluorescent intensity. b Western blot analysis of Ph-negative US7, Ph-positive TXL2, relapse LAX56 and diagnosis LAX57 BP-ALL patient-derived lines (Genetex Galectin-1 Abs). c Real-time RT/PCR for Galectin-1 mRNA on the indicated ALLs cultured for 24 h in complete medium but without OP9 stroma, then stimulated with the indicated recombinant proteins for an additional 24 h. Two samples represent ALL cells grown with irradiated OP9 stroma as indicated. Values shown were normalized to a reference gene, GAPDH, and are comparisons with non-treated US7 cells. One of two experiments, similar results. US7 GST-Gal3 compared to GST *p < 0.05. 95% CI, 1-way ANOVA. d FACS analysis for Galectin-1 and Galectin-3 with and without cross-linking with DTSSP to stabilize Galectin-1 and Galectin-3 protein complexes on the cell surface. Antibodies used are indicated above the panels. e FACS analysis (antibodies- R&D Systems Galectin-1) for cell surface and total Galectin-1 expression in the indicated ALLs co-cultured on OP9 stromal cells mitotically inactivated by mitomycin C treatment. Percentages indicated are for the lower right-hand quadrant. f Galectin-1 levels measured by ELISA consisting of GeneTex anti-Galectin-1 capture antibodies and Abcam anti-Galectin-1 primary detection antibodies with secondary HRP-conjugated goat anti-mouse antibodies. Values represent ng/ml in undiluted control and ALL human plasma samples (n = 5–6 for each) from PB and BM collected with anticoagulant after removal of the cellular component