Fig. 3

Overexpression of TUSC7 or inhibition of miR-224 inhibited proliferation of ESCC cells and promoted cell apoptosis. a-c EC9706 or KYSE30 cells were transfected with pcDNA or pcDNA-TUSC7. MTT assay showed that overexpression of TUSC7 inhibited proliferation of ESCC cells (a). Colony formation assay showed that overexpression of TUSC7 inhibited colony formation (b). Flow cytometry showed that overexpression of TUSC7 promoted apoptosis of ESCC cells (c). D-E. EC9706 or KYSE30 cells were transfected with NC or miR-224 inhibitor. qRT-PCR showed that miR-224 expression was downregulated (d). MTT assay showed that miR-224 inhibitor inhibited proliferation of ESCC cells (e). Colony formation assay showed that miR-224 inhibitor inhibited colony formation (f). Flow cytometry showed that miR-224 inhibitor promoted apoptosis of ESCC cells (g)