Fig. 1

The soluble cytokines secreted by leukemic cells contribute to the adipocyte remodeling. a BMSC-derived adipocytes cultured with HG-DMEM supplied with 10% FBS (Ctr) or with the conditioned medium (CM) of healthy mononuclear cells (MNC CM), primary leukemic cells (LC CM), THP-1 cell line (THP-1 CM), K562 cell line (K562 CM), HL-60 cell line (HL-60 CM) and Kasumi cell line (Kasumi CM) for 5 days. Adipocytes were stained by ORO. Both images are at a magnification of 400×. b The average area of adipocytes cultured with the CM of different AML cells were compared with the controls by using Image-Pro-Plus 5.1. *P < 0.05, **P < 0.01. c The content of adipocyte lipid-droplets in indicated groups was detected by OD values after ORO staining. *P < 0.05, **P < 0.01. d RT-qPCR analysis of adipogenic genes (FABP4, C/EBPα and PPARγ) in indicated groups. *P < 0.05, **P < 0.01. Results shown are from three independent experiments. Values shown are the mean ± SEM