Fig. 8

Downregulation of SGK1 inhibits metastasis in vivo. Briefly, mice were injected via tail vein with 1 × 10⁶ PC3^LV2-Ctrl cells or 1 × 10⁶ PC3^shSGK1 cells suspended in 0.1 ml PBS. Mice were euthanized at 8 weeks post-implantation, lungs were obtained and shown (a). b Lungs were fixed in 10% formal dehyde, and hematoxylin and eosin (HE) stained for metastatic nodules, red arrow indicates metastatic nodule. (c) Representative immunofluorescence images of PSA expression in lung tissue. Representative images are presented, scale bar, 100 μm