Fig. 2

Kinetics and drug sensitivity of tumor spheroids from primary HCC cells. a Kinetics of tumor spheroids from AMC-H1 and AMC-H2 cells at the indicated times. Cells were cultured in 96-well ultra-low attachment (ULA) plates for 96 h. b Drug sensitivities in tumor spheroids of AMC-H1 and AMC-H2 HCC cells. Cells were cultured in 96-well ULA plates for 3 day and then treated with 5-FU, cisplatin, and sorafenib at the indicated concentrations for 7 day. Then, tumor spheroids were stained with ethidium homodimer-1 (EthD-1; left panel), and the staining intensity was analyzed (right panel). All images were obtained and analyzed using the HCS system. Data are shown as the mean ± SD of triplicate experiments. Scale bar = 200 μm. *p < 0.05, **p < 0.005