Fig. 5

NKG2D and DNAM-1 play a role in chemotherapy-induced sensitivity to DNTs. a & b Representative histograms of the expression of NKG2D ligands (MIC-A/B, ULBP4) and DNAM-1 ligands (CD112, CD155) are shown. a KG1a or b PBMCs from an HD were treated with media (dark grey), AraC (grey), and DNR (light grey) and then analysed for surface expression of NKG2D and DNAM-1 ligands by flow cytometry. Bolded numbers represent the mean fluorescence intensity of each stain. c KG1a were treated with 0.25 μg/mL AraC or 0.4 μg/mL DNR for 24 h and used as targets in a blocking assay. DNTs were incubated with anti-NKG2D (10 μg/mL) and anti-DNAM-1 (10 μg/mL) or 20 μg/mL of the isotype for 30 min and then washed with media prior to co-culture with chemotherapy pre-treated KG1a at an E:T ratio of 4:1. These experiments were conducted independently 3 times. **, p < 0.01; ***, p < 0.001