Fig. 5

TRAF6 plays an oncogenic role in tumorigenesis of human oral cancer cells in vivo. a TRAF6-Akt signaling pathway and p-p65 protein expression in SAS cells transfected with TRAF6 shRNA. b Cell growth curve resulting from TRAF6 shRNA in SAS cells. *p < 0.05, versus control. c Measurement of body weight in NOD/SCID mice once per week. d Measurement of tumor weight of SAS xenograft in NOD/SCID mice. e SAS xenograft tumor growth curves in NOD/SCID nude mice. SAS cells silenced with TRAF6 shRNAs were injected into nude mice (n = 5 for each group) and monitored for tumorigenesis. ** p < 0.01 versus untreated controls. f Direct observation of mice with tumors from the control and TRAF6 shRNAs groups. g Analysis of the TRAF6-Akt signaling pathway related proteins from tumors transfected with TRAF6 shRNA and grown in NOD/SCID mice by western blot, and (h) H/E staining and immunohistochemical staining for analysis of TRAF6-positive cells (brown)