Fig. 4

miR-221 induces proliferative effects in CAFs, MDA-MB 231 and SkBr3 cells. Cell proliferation in (a) CAFs, (d) MDA-MB 231 and (g) SkBr3 cells transfected every 2 days with 25 nM miR-Ctrl and 25 nM miR-221, alone or in combination with 100 nM LNA-i-miR-221 (LNA-i) and then counted on day 6. Cell proliferation in CAFs (b) MDA-MB 231 (e) and SkBr3 (h) cells transfected with shRNA or shRel for 8 h and then transfected for 48 h with 25 nM miR-221. Cell proliferation in CAFs (c), MDA-MB 231 (f) and SkBr3 (i) cells transfected with shRNA or shCTGF for 8 h and then transfected for 48 h with 25 nM miR-221. The transfections were renewed every 2 days and cells were counted on day 6. Each data point is the mean ± SD of three independent experiments performed in triplicate. (**) indicates p < 0.01