Fig. 6

miR-221 triggers migratory effects in CAFs, MDA-MB 231 and SkBr3 cells. Cell migration as evaluated by Boyden Chamber assay in (a) CAFs, (c) MDA-MB 231 and (e) SkBr3 cells transfected for 48 h with 25 nM miR-Ctrl and 25 nM miR-221, alone or in combination with 100 nM LNA-i-miR-221 (LNA-i). Cell migration, evaluated by Boyden Chamber assay, in CAFs (b) MDA-MB 231 (d) and SkBr3 (f) cells transfected with shRNA or shRel for 8 h and then transfected for 48 h with 25 nM miR-221. Cells were counted in at least 10 random fields at 10× magnification (Scale bar =200 μm) in three independent experiments performed in triplicate. (**) indicates p < 0.01