Fig. 7

Co-treatment of olaparib and doxorubicin increases apoptosis of osteosarcoma cells. a Flow cytometry analysis with annexin V and propidium iodide staining in U2OS, SaOS2, and MG63 cells showed a significant increase of apoptotic population (early apoptosis; annexin V⁺/PI⁻, late apoptosis; annexin V⁺/PI⁺) with co-treatment of 10 μM olaparib and 0.2 μM doxorubicin. Flow cytometry analysis was performed 72 h after treatment of olaparib and/or doxorubicin. b The expression of cleaved PARP1, cleaved caspase 3, and BAX increased and the expression of BCL2 was decreased with co-treatment of olaparib and doxorubicin as demonstrated by western blot analysis. *, versus control, P < 0.05; **, versus control, P < 0.001; †, versus doxorubicin, P < 0.05