Fig. 2

a The protein levels of FGF15 in serum, ileum and liver tissue by ELISA assays. b the FGF15 mRNA levels and protein levels from 4 groups at month 10. The lysates of ileal tissues from fasting and non-fasting wild type mice are used as positive controls for FGF15, while the lysate of H4IIE cells, a rat hepatoma cell line, is used as a negative control because rats do not express FGF15. N-fasting: non-fasting. c Representative images of FGF15 protein distribution in hepatic parenchyma and computer image-quantification in all 4 experimental groups at month 10 by immunohistochemical staining. Ileal tissues from fasting and non-fasting wild type mice are used as positive controls for FGF15 IHC staining. N-fasting: non-fasting. UT: untreated; CD: control diet; HFD: high fat diet; DEN: N-nitrosodiethylamine. *, P < 0.05 vs UT + CD