Fig. 3

a-c Representative images of IHC for FGFR4, β-klotho, and FASN in hepatic tissues and protein expression by computer image-quantification, and Western blot analysis of protein levels in the 4 experimental groups at month 10. The protein expression by computer image-quantification presented as Average Threshold Area (Average Th Area). The protein levels of Western blot analysis were quantified by image analysis and presented as pixel ratio over the control GAPDH. KLB: β-klotho; UT: untreated; CD: control diet; HFD: high fat diet; DEN: N-nitrosodiethylamine. *, P < 0.05 vs UT + CD. d mRNA expressions (CD36, PPAR-α and PPAR-γ) in the liver tissues from 4 groups at month 6 and month 10. Western blot analysis of FGF15 protein levels in three HCC cell lines (Hepal-6, HepG2 and H4IIE) challenged by FFA 0–72 h. Western blot analysis were quantified by image analysis and presented as pixel ratio over the control GAPDH. e The protein levels of FGF15 and FGF19 in the supernatant of Hepa1–6, H4IIE, and HepG2 cells by ELISA assays. *, P < 0.05; **, P < 0.01