Fig. 5
a Time-course study of relative mRNA levels of E-Cadherin and vimentin in hepal-1 cells treated by FFA. The representative images of E-Cadherin and vimentin in hepal-1 cells treated by FFA at 72 h. b A trans-well migration assay to study the migratory ability of Hepal-6 cells in response to FFA treatment. Migration cell number was calculated. c A scratch-wound healing assay to study the migratory ability of Hepal-6 cells in response to FFA treatment. A 250 μm scratch-wound was made on 90% confluent cells in each treatment group. Average length of scratch-wound was calculated in each treatment group and the migratory ability of cells was represented as wound closure length. *: P < 0.05 vs 0 h. d Western blot analysis of EpCAM, β-catenin, FGFR4 and FASN in hepal-1 cells treated by FFA. The protein levels were quantified by image analysis and presented as pixel ratio over the control GAPDH. #: P < 0.05 vs 0 h. FFA: free fatty acid; h: hour. UT: untreated; BLU: BLU9931; XAV: XAV 939. e-f siPPARa and siCD36 as well as the scrambled siRNA were applied to the Hapal-6 and HepG2 cells for 48 h to silence the mRNA of PPAR-α and CD36, and the cells were challenged by FFA for 48 h. Western blot analysis was performed to determine the protein levels of FGF15, FGF19, FGFR4, EpCAM, β-catenin, andcyclin D. 1: scrambled siRNA; 2: FFA; 3: FFA+ siCD36; 4: FFA+ siPPARa; 5: FFA+ siCD36 + siPPARa. N.S.: no significant importance; *, P < 0.05; **, P < 0.01