Fig. 3

Effects of Lycorine on cell viability of GBM cells holding different EGFR status. a Expression of EGFR mRNA on different GBM cell lines. Surface EGFR expression on glioma cell lines (U87, wild type EGFR; LN229, wild type EGFR amplification, U251, wild type EGFR amplification, A172, EGFRvIII mutant, Gli36vIII, EGFRvIII mutant, GBM6, wild type EGFR and EGFRvIII co-existing) and a healthy normal human IMA2.1 astrocytes was monitored by RT-PCR. b Lycorine suppresses GBM cell’s proliferation independent of EGFR mutation status but dependent of EGFR expression level. The cell viability assay stained by SRB was performed as described in Methods. All data are represented as mean ± S.D. from triplicate wells