Fig. 3

Supervillin regulates hypoxia-induced epithelial-mesenchymal transition (EMT) of HCC. a. Bright-field microscopy showing the morphological changes that occur when HepG2 and Huh-7 cells are cultured for 16 h in hypoxia. b. Immunoblots showing the changes in E-cadherin, Vimentin, Snail1, and supervillin isoforms during hypoxia after treatment with supervillin-specific siRNA. HepG2 and Huh-7 cells were transfected with a control or supervillin-specific siRNA and incubated under normoxia for 48 h followed by being scratched and exposed to normoxia or hypoxia for 16 h; β-tubulin was used as the loading control. c. Immunofluorescence staining showing that the relative amounts and localization of E-cadherin, Snail1, and Vimentin under normal and hypoxic conditions in Huh-7 cells treated with control or supervillin-specific siRNA. Scale bar = 50 μm